UNIVERSITY OF CALIFORNIA, IRVINE
Environmental Health and Safety

 

Laboratory Safety Guidelines 2005-2006

 

 

SECTION 1: HEALTH AND SAFETY IN THE RESEARCH LABORATORY

 

Introduction 1-1

 

EH&S Programs 1-1

Training Resources..... 1-3

 

 

SECTION 2: CHEMICAL HAZARDS - RECOGNITION, EVALUATION AND CONTROL

 

Recognition of Chemical Hazards........................ 2-1

 

Evaluation of Chemical Hazards 2-1

Material Safety Data Sheets (MSDS)......... 2-1

Health Effects of Chemicals...... 2-2

Threshold Limits Values............ 2-3

Dose-Response Relationship. 2-3

Routes of Entry 2-3

Target Organ Effects........... 2-3

 

Control of Chemical Hazards........................ 2-4

Designate Area 2-4

Engineering Control.......... 2-4

Personal Protective Equipment (PPE)........................ 2-5

Chemical Hygiene Plan (CHP).... 2-6

Standard Operating Procedures (SOP)........................ 2-7

Work Practice Controls........ 2-7

Chemical Storage........................ 2-9

Chemical Waste 2-11

 

 

SECTION 3: PHYSICAL HAZARDS - RECOGNITION, EVALUATION AND CONTROLS

 

Recognition of Physical Hazards........................ 3-1

 

Evaluation of Physical Hazards........................ 3-1

Compressed Gases............. 3-1

Vacuum Operations.... 3-3

Electrical Safety 3-4

Ergonomics………………………………………. 3-5

Machinery Equipment..... 3-8

Centrifuges... 3-8

Research Animals........................ 3-9

Glassware Safety...................... 3-10

Temperature Extremes...... 3-10

Oil & Sand Baths...................... 3-10

Pressure Vessels...................... 3-11

Control Methods of Physical Hazards....... 3-11

 

 

SECTION 4: BIOLOGICAL HAZARDS - RECOGNITION, EVALUATION AND CONTROL

 

Recognition of Biological Hazards......... 4-1

Biohazards.... 4-1

 

Evaluation of Biological Hazards......... 4-1

Biosafety Level 4-1

Waste............ 4-3

 

Control of Biological Hazards......... 4-4

Working with Biohazards.... 4-4

Biosafety Cabinets........ 4-5

Handling Waste........................ 4-7

 

 

SECTION 5: RADIOACTIVE HAZARDS - RECOGNITION, EVALUATION AND CONTROL

 

Ionizing Radiation....... 5-1

Recognition of Ionizing Radiation....... 5-1

Evaluation of Ionizing Radiation Hazards......... 5-1

Control Methods for Ionizing Radiation Hazards......... 5-1

 

Non-Ionizing Radiation....... 5-3

Recognition of Non-Ionizing Radiation Hazards......... 5-3

Evaluation of Non-Ionizing Radiation

Hazards......... 5-3

Control Methods for  Non-Ionizing Radiation Hazards......... 5-4

 

UV Radiation 5-4

IR Radiation.. 5-4

Radio-frequency (RF) & Microwave (MW)

Radiation....... 5-4

Laser Radiation 5-4

Ten Most Common Causes of Laser-Produced

Eye Injuries... 5-6

 

 

SECTION 6: EMERGENCIES

 

Fire Incidents 6-1

Fire Safety..... 6-1

In the Event of a Fire................ .6-2

Fire Extinguishers........................ 6-3

 

Chemical Spills 6-5

Respond to Chemical Spills 6-5

 

Lab Injuries... 6-7

First Aid........ 6-8

 

Basic First Aid For Chemical Exposure....... 6-8

Eye Contact.. 6-8

Skin Contact. 6-8

Inhalation...... 6-8

 

What to Do in a Medical Emergency.... 6-9

 

Handling Medical Emergencies in a Classroom Setting......... 6-10

 

APPENDICES

Appendix A

Responsibilities

Safety on Site (SOS)

 

Appendix B

Injury and Illness Prevention

 

Appendix C

Chemical Hygiene Plan (CHP)

 

Appendix D

Standard Operating Procedure (SOP)

 

Appendix E

Classes of Hazardous Materials

 

Appendix F

Formaldehyde

 

Appendix G

Chemical Incompatibility Table

 

Appendix H

Target Organ Categorization

 

Appendix I

Personal Protective Equipment (PPE)

 

Appendix J

Gloves - Chemical Resistance and Selection

Appendix K

Spill Clean-Up

 

Appendix L

Labeling of Hazardous Materials

 

Appendix M

Chemical Compatibility & Storage of Hazardous Materials

 

Appendix N

Recommended Biosafety Levels

 

Appendix O

Biosafety Cabinets

 

Appendix P

Autoclaves

Appendix Q

Controlled Substances

Appendix R

Shipping of Dangerous Goods

 

Appendix S

Field Safety

 

Appendix T

Glossary

 

 

INDEX

 

Back Cover: INFORMATION & ASSISTANCE