UNIVERSITY OF CALIFORNIA, IRVINE
Environmental Health and Safety
Laboratory Safety Guidelines 2005-2006
SECTION 1: HEALTH
AND SAFETY IN THE RESEARCH LABORATORY
Introduction 1-1
EH&S Programs 1-1
Training Resources..... 1-3
SECTION 2: CHEMICAL
HAZARDS - RECOGNITION, EVALUATION AND CONTROL
Recognition of Chemical
Hazards........................ 2-1
Evaluation of Chemical
Hazards 2-1
Material Safety Data
Sheets (MSDS)......... 2-1
Health Effects of
Chemicals...... 2-2
Threshold Limits Values............ 2-3
Dose-Response
Relationship. 2-3
Routes of Entry 2-3
Target Organ Effects........... 2-3
Control of Chemical
Hazards........................ 2-4
Designate Area 2-4
Engineering Control.......... 2-4
Personal Protective
Equipment (PPE)........................ 2-5
Chemical Hygiene Plan
(CHP).... 2-6
Standard Operating Procedures
(SOP)........................ 2-7
Work Practice Controls........ 2-7
Chemical Storage........................ 2-9
Chemical Waste 2-11
SECTION 3: PHYSICAL
HAZARDS - RECOGNITION, EVALUATION AND CONTROLS
Recognition of Physical
Hazards........................ 3-1
Evaluation of Physical
Hazards........................ 3-1
Compressed Gases............. 3-1
Vacuum Operations.... 3-3
Electrical Safety 3-4
Ergonomics
.
3-5
Machinery Equipment..... 3-8
Centrifuges... 3-8
Research Animals........................ 3-9
Glassware Safety...................... 3-10
Temperature Extremes...... 3-10
Oil & Sand Baths...................... 3-10
Pressure Vessels...................... 3-11
Control Methods of
Physical Hazards....... 3-11
SECTION 4:
BIOLOGICAL HAZARDS - RECOGNITION, EVALUATION AND CONTROL
Recognition of
Biological Hazards......... 4-1
Biohazards.... 4-1
Evaluation of Biological
Hazards......... 4-1
Biosafety Level 4-1
Waste............ 4-3
Control of Biological
Hazards......... 4-4
Working with Biohazards.... 4-4
Biosafety Cabinets........ 4-5
Handling Waste........................ 4-7
SECTION 5: RADIOACTIVE
HAZARDS - RECOGNITION, EVALUATION AND CONTROL
Ionizing Radiation....... 5-1
Recognition of Ionizing
Radiation....... 5-1
Evaluation of Ionizing
Radiation Hazards......... 5-1
Control Methods for
Ionizing Radiation Hazards......... 5-1
Non-Ionizing Radiation....... 5-3
Recognition of Non-Ionizing
Radiation Hazards......... 5-3
Evaluation of
Non-Ionizing Radiation
Hazards......... 5-3
Control
Methods for Non-Ionizing Radiation
Hazards......... 5-4
UV Radiation 5-4
IR Radiation.. 5-4
Radio-frequency (RF)
& Microwave (MW)
Radiation....... 5-4
Laser Radiation 5-4
Ten Most Common Causes
of Laser-Produced
Eye Injuries... 5-6
Fire Incidents 6-1
Fire Safety..... 6-1
In the Event of a Fire................ .6-2
Fire Extinguishers........................ 6-3
Chemical Spills 6-5
Respond to Chemical
Spills 6-5
Lab Injuries... 6-7
First Aid........ 6-8
Basic First Aid For
Chemical Exposure....... 6-8
Eye Contact.. 6-8
Skin Contact. 6-8
Inhalation...... 6-8
Handling Medical
Emergencies in a Classroom Setting......... 6-10
APPENDICES
Responsibilities
Safety on Site (SOS)
Injury and Illness
Prevention
Chemical Hygiene Plan
(CHP)
Standard Operating
Procedure (SOP)
Formaldehyde
Chemical Incompatibility
Table
Target Organ
Categorization
Personal Protective
Equipment (PPE)
Gloves - Chemical Resistance and Selection
Labeling of Hazardous
Materials
Recommended Biosafety Levels
Biosafety Cabinets
Autoclaves
Controlled Substances
Shipping of Dangerous
Goods
Field Safety
Glossary
Back Cover: INFORMATION &
ASSISTANCE